Although bacterial viruses, or bacteriophages, are present in many natural environments, they cannot survive autonomously. They require a host cell to reproduce and survive. In this experiment, students will learn about the different life cycles of bacterial viruses. They will then perform a viral plaque assay to indirectly visualize the viruses and to determine viral titer and multiplicity of infection (MOI).
Kit includes: instructions, cells, phage beads, Ready-Pour Agar, colourTOP™ Agar, microtest tubes, sterile loops, petri plates, luria broth, PBS
All you need: 37˚C incubation oven, waterbath, automatic micropipets with tips
For: 10 groups.
10 lab groups
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